Journal: Open Medicine

Article Title: Mechanisms of hesperetin in treating metabolic dysfunction-associated steatosis liver disease via network pharmacology and in vitro experiments

doi: 10.1515/med-2025-1215

Figure Lengend Snippet: Pattern diagram of molecular docking: (a) Hesperetin-IL-6, (b) hesperetin-CYCS, (c) hesperetin-TGFB1, (d) hesperetin-IL1B, (e) hesperetin-SOCS3, and (f) hesperetin-CEPBA.

Article Snippet: The primary antibodies against interleukin-6 (IL-6) (DF6087), p-JAK2 (AF3024), JAK2 (AF6022), p-STAT3 (AF3293), and STAT3 (AF6294) were purchased from Affinity Biosciences (OH, USA).

Techniques:

Journal: Open Medicine

Article Title: Mechanisms of hesperetin in treating metabolic dysfunction-associated steatosis liver disease via network pharmacology and in vitro experiments

doi: 10.1515/med-2025-1215

Figure Lengend Snippet: Effects of hesperetin on IL-6-STAT3-SOCS3 pathway in FFA-induced HepG2 cells. (a) IL-6 content in cell culture medium. (b) Relative protein levels of IL-6 in HepG2 cells. (c) Relative protein levels of p-STAT3/STAT3 and SOCS3 in HepG2 cells. (d) Glucose absorption in different groups. (e) Levels of IL-6 in HepG2 cells. (e) Relative protein levels of p-STAT3/STAT3 and SOCS3 in IL-6-induced HepG2 cells. The data represented as mean ± SD, n = 3. * P < 0.05, ** P < 0.01, compared to Ctrl; # P < 0.05, ## P < 0.01, compared to FFA.

Article Snippet: The primary antibodies against interleukin-6 (IL-6) (DF6087), p-JAK2 (AF3024), JAK2 (AF6022), p-STAT3 (AF3293), and STAT3 (AF6294) were purchased from Affinity Biosciences (OH, USA).

Techniques: Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characteristics of JAK2 and Its Effect on the Milk Fat and Casein Synthesis of Ovine Mammary Epithelial Cells

doi: 10.3390/ijms25074027

Figure Lengend Snippet: Cloning and nucleotide sequence analysis of ovine JAK2 . ( A ) Agarose gel electrophoresis detection of the cloned coding sequence (CDS) region of ovine JAK2 . M: DL5000 DNA marker; 1–4: ovine JAK2 sequences. ( B ) Comparison of cloned sequences obtained in this study with predicted ovine JAK2 sequences (XM_042243421.2). ( C ) Comparison of nucleotide homology between sequences obtained in this study and JAK2 sequences from other animal species. ( D ) The neighbor-joining tree of sequences obtained in this study and JAK2 sequences identified in other animal species. The cloned sequence in this study is shown in a box and JAK2 sequences from other animal species include goats (XM_01805202), cattle (XM_003586385.6), yaks (XM_005891363.2), pigs (NM_214113.1), humans (NM_001322194.2), rhesus monkeys (NM_001265901.2), chimpanzees (XM_001139368.6), mice (NM_001048177.3), and chickens (NM_001030538.3).

Article Snippet: After 30 min, the tissues were incubated with rabbit primary antibody against JAK2 (1: 100; Abmart, Shanghai, China) at 4 °C overnight.

Techniques: Clone Assay, Sequencing, Agarose Gel Electrophoresis, Marker, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characteristics of JAK2 and Its Effect on the Milk Fat and Casein Synthesis of Ovine Mammary Epithelial Cells

doi: 10.3390/ijms25074027

Figure Lengend Snippet: The characteristic analysis of the ovine JAK2 protein. ( A ) Amino acid composition analysis of the ovine JAK2 protein. ( B ) Hydrophilic and hydrophobic cluster analysis of the ovine JAK2 protein.

Article Snippet: After 30 min, the tissues were incubated with rabbit primary antibody against JAK2 (1: 100; Abmart, Shanghai, China) at 4 °C overnight.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characteristics of JAK2 and Its Effect on the Milk Fat and Casein Synthesis of Ovine Mammary Epithelial Cells

doi: 10.3390/ijms25074027

Figure Lengend Snippet: Immunofluorescence staining results of the JAK2 protein in ovine mammary gland tissue.

Article Snippet: After 30 min, the tissues were incubated with rabbit primary antibody against JAK2 (1: 100; Abmart, Shanghai, China) at 4 °C overnight.

Techniques: Immunofluorescence, Staining

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characteristics of JAK2 and Its Effect on the Milk Fat and Casein Synthesis of Ovine Mammary Epithelial Cells

doi: 10.3390/ijms25074027

Figure Lengend Snippet: The relative expression level of JAK2 in six ovine tissues. Different lowercase letters above the bars represent significant differences ( p < 0.05).

Article Snippet: After 30 min, the tissues were incubated with rabbit primary antibody against JAK2 (1: 100; Abmart, Shanghai, China) at 4 °C overnight.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characteristics of JAK2 and Its Effect on the Milk Fat and Casein Synthesis of Ovine Mammary Epithelial Cells

doi: 10.3390/ijms25074027

Figure Lengend Snippet: Effect of JAK2 on viability of ovine mammary epithelial cells (OMECs). ( A , B ) Relative expression levels of JAK2 when pcDNA3.1- JAK2 and three groups of small interfered JAK2 were transfected into OMECs. ( C ) Effect JAK2 on the viability of OMECs, detected using a CCK-8 assay. ** p < 0.01.

Article Snippet: After 30 min, the tissues were incubated with rabbit primary antibody against JAK2 (1: 100; Abmart, Shanghai, China) at 4 °C overnight.

Techniques: Expressing, Transfection, CCK-8 Assay

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characteristics of JAK2 and Its Effect on the Milk Fat and Casein Synthesis of Ovine Mammary Epithelial Cells

doi: 10.3390/ijms25074027

Figure Lengend Snippet: Effect of JAK2 on the proliferation of ovine mammary epithelial cells (OMECs). ( A ) Effect of JAK2 on the number of proliferated OMECs, detected using an EdU assay. Scale bar, 200 µm. ( B ) The percentage of EdU-labeled positive OMECs was calculated using ImageJ 1.54d software. ( C ) The expression levels of the cell proliferation marker genes CDK2 , CDK4 , and PCNA when pcDNA3.1- JAK2 and si- JAK2- 1 were transfected into OMECs. * p < 0.05 and ** p < 0.01.

Article Snippet: After 30 min, the tissues were incubated with rabbit primary antibody against JAK2 (1: 100; Abmart, Shanghai, China) at 4 °C overnight.

Techniques: EdU Assay, Labeling, Software, Expressing, Marker, Transfection

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characteristics of JAK2 and Its Effect on the Milk Fat and Casein Synthesis of Ovine Mammary Epithelial Cells

doi: 10.3390/ijms25074027

Figure Lengend Snippet: The triglyceride contents ( A ) and the expression levels of four milk fat synthesis marker genes ( B ) when pcDNA3.1- JAK2 or si- JAK2 -1 were transfected into ovine mammary epithelial cells (OMECs). * p < 0.05 and ** p < 0.01.

Article Snippet: After 30 min, the tissues were incubated with rabbit primary antibody against JAK2 (1: 100; Abmart, Shanghai, China) at 4 °C overnight.

Techniques: Expressing, Marker, Transfection

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characteristics of JAK2 and Its Effect on the Milk Fat and Casein Synthesis of Ovine Mammary Epithelial Cells

doi: 10.3390/ijms25074027

Figure Lengend Snippet: The expression levels of four casein genes when pcDNA3.1- JAK2 or si- JAK2 -1 were transfected into ovine mammary epithelial cells (OMECs). ** p < 0.01.

Article Snippet: After 30 min, the tissues were incubated with rabbit primary antibody against JAK2 (1: 100; Abmart, Shanghai, China) at 4 °C overnight.

Techniques: Expressing, Transfection

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characteristics of JAK2 and Its Effect on the Milk Fat and Casein Synthesis of Ovine Mammary Epithelial Cells

doi: 10.3390/ijms25074027

Figure Lengend Snippet: Primer sequence information used for RT–qPCR.

Article Snippet: After 30 min, the tissues were incubated with rabbit primary antibody against JAK2 (1: 100; Abmart, Shanghai, China) at 4 °C overnight.

Techniques: Sequencing, Amplification

Journal: International Journal of Molecular Sciences

Article Title: Molecular Characteristics of JAK2 and Its Effect on the Milk Fat and Casein Synthesis of Ovine Mammary Epithelial Cells

doi: 10.3390/ijms25074027

Figure Lengend Snippet: The siRNA sequences of JAK2 .

Article Snippet: After 30 min, the tissues were incubated with rabbit primary antibody against JAK2 (1: 100; Abmart, Shanghai, China) at 4 °C overnight.

Techniques: